Journal: Experimental and Molecular Pathology

Article Title: Shallow whole genome sequencing for robust copy number profiling of formalin-fixed paraffin-embedded breast cancers

doi: 10.1016/j.yexmp.2018.03.006

Figure Lengend Snippet: Features of input DNA and libraries generated from FFPE blocks collected at three different sites. Data provided in minimum-maximum range and median in brackets.

Article Snippet: We tested two kits (Illumina FFPE TruSEQ kit and Rubicon Genomics Thruplex DNASeq) using four FFPE samples to generate sequencing libraries and found comparable results ( a–b).

Techniques: Generated

Journal: Experimental and Molecular Pathology

Article Title: Shallow whole genome sequencing for robust copy number profiling of formalin-fixed paraffin-embedded breast cancers

doi: 10.1016/j.yexmp.2018.03.006

Figure Lengend Snippet: Measured standard deviations from the QDNASEQ copy number plots and associations with the quality of sequencing libraries. A. Bar charts showing proportion of samples with different input DNA quality (based on ΔCt) in each sequencing quality group. B. Bar charts showing proportion of samples from FFPE blocks of different fragment sizes in each sequencing quality group. C. Bar charts showing proportion of samples with different amount of input DNA in each sequencing quality group. VG = very good, G = good, I = intermediate, P = poor, F = fail.

Article Snippet: We tested two kits (Illumina FFPE TruSEQ kit and Rubicon Genomics Thruplex DNASeq) using four FFPE samples to generate sequencing libraries and found comparable results ( a–b).

Techniques: Sequencing